![]() ![]() The R 2 value for the dilution series (0.9997) is evidence that accuracy and reproducibility can be achieved even for high concentration samples read without dilution. A highly concentrated preparation of plasmid DNA at 12.8 µg/µL was run on the Lunatic in a dilution series to demonstrate the high linearity of the data generated by the apparatus. ![]() Lunatic was a great solution for the group because it enabled samples to be read without the need for dilution with the desired accuracy for downstream purposes. One group experienced inconsistent results with their previous quantification method for their high-concentration plasmid preparations, which required large sample dilutions. In the webinar, Nelis discusses specific customer use cases where Lunatic has been successful in resolving discrepancies and pain points. One example of Lunatic performance is shown through absorbance readings of uracil and tryptophan standards across 5 different instruments, run in octuplicate, which were found to be within 1.65% of the certified value, with the mean and the median within 1.25%, demonstrating measurement reproducibility and accuracy. To demonstrate instrument performance, several applications on Lunatic measure absorbance standards certified by the National Institutes of Standards and Technology which validate accuracy, precision, dynamic range, and more. Capable of running 96 samples in ≤10 minutes with only 2 µL of sample, this unique platform has a wide dynamic range of 0.03-275 OD (0.5 – 13750 ng/µL dsDNA), which eliminates the need for sample dilution. The system is a full spectrum UV/Vis spectrophotometer with 96-well plates containing built-in microfluidic circuits and fixed measurement pathlengths. In this webinar, Nelis Denys, Product Manager for Lunatic & Stunner, outlines the benefits of Lunatic and provides use-case examples for quantification of nucleic acids. Lunatic from Unchanined Labs provides a solution to these challenges by providing fast, simple and accurate DNA quantification. Quantifying nucleic acids by measuring UV absorbance with spectrophotometry has long been established in the lab, but now newer technologies are overcoming classic limitations to make UV absorbance a more complete solution. For most traditional tools, the list of limitations is long: low throughput, high sample volumes, limited dynamic range, expensive dyes, inaccuracy from contaminants, and more. In nucleic acid quantification, the limitations of traditional analytical tools put different hurdles in the way of research any time quantification is needed. Job Title: Product Manager, Lunatic & Stunner
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |